Next-Generation Protein Sequencing™ Resolves Peptide Variants Derived from Tropomyosin Proteoforms

Next-Generation Protein Sequencing (NGPS) resolves proteotypic representing the three main types of regulation — genetic, splicing, and post-translational modifications — contributing to proteomic molecular diversity. Kinetic signatures produced by NGPS are a novel orthogonal datatype that can complement other commonly used proteomics approaches. NGPS data can be used for a range of protein characterization applications, including detecting tissue-specific variant expression, epitope mapping, and PTM profiling.