Empower a New Era of Biomarker Development on Platinum®
Uncover disease biomarkers with the precision of single-molecule protein sequencing.
Now you can interrogate disease biomarkers using our advanced Next-Generation Protein Sequencer.™
Learn More About PlatinumWhy choose Platinum for biomarker development?
Biomarker development is essential for drug development, early disease detection, personalized therapies, and gaining a deeper understanding of disease progression. When it comes to biomarker identification, choosing our platform offers researchers a comprehensive solution that unlocks deeper insights into the proteome.
Platinum’s single-molecule protein sequencing technology allows for more detailed and accurate analysis of a wide range of proteins, revealing critical biomarkers that may go undetected with conventional approaches. Platinum makes it easier to validate potential biomarkers and bring precision medicine closer to reality. With integrated informatics for simplifying complex data analysis, our platform provides a scalable solution that grows with your research, supporting both current projects and future advancements.
Harness single-molecule sequencing and seamless workflow integration to:
Characterize biomarkers identified in high-throughput proteomic screening assays
Reveal biomarkers that go undetected with conventional methods
Identify binding partners in enrichment techniques such as IP, co-IP, and chromatography
Enable multiomics research by seamlessly integrating proteomics with genomics and transcriptomics
Explore our resources to discover how Platinum can enhance your biomarker development:
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Protein Sequencing With Single-Amino Acid Resolution Discerns Peptides That Discriminate Tropomyosin Proteoforms
Protein variants of the same gene–proteoforms–can have high molecular similarity yet exhibit different biological functions. Thus, identifying unique peptides that unambiguously map to proteoforms can provide crucial biological insights. In humans, four human tropomyosin (TPM) genes produce similar proteoforms that can be challenging to distinguish with standard proteomics tools.
Dr. Gloria Sheynkman and her team at the University of Virginia School of Medicine have published a preprint on BioRxiv showcasing the capabilities of the Platinum® Next-Generation Protein Sequencer™ in advancing their research in this complex area of study.
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Quantum-Si’s Next Generation Protein Sequencing Technology Enables Rapid and Accurate Distinction of Variants of the SARS-CoV-2 Virus – APP NOTE
The application note highlights Quantum-Si’s protein sequencing technology on the Platinum instrument for swift and accurate differentiation of SARS-CoV-2 variants based on the amino acid sequences of their Spike proteins. This identification and monitoring of variants facilitate a better understanding of their public health impact and contribute to the development of effective treatments and vaccines.
Download the application note to learn more about this groundbreaking technology.
By scrutinizing a critical mutation, we identified distinct kinetic impacts on the binding of recognizers. These disparities in pulse durations (PD) effectively illustrate the Platinum instrument’s proficiency in differentiating among the Alpha, Delta, and Omicron variants through the lens of a single mutational change.
In this analysis, we focus on isolating the Omicron variant from the Alpha and Delta variants by identifying a singular, characteristic peptide. This specialized capability augments the accuracy and precision with which SARS-CoV-2 variants can be differentiated using Quantum-Si’s Platinum instrument.
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Enrichment and Sequencing of Albumin Protein from Urine – APP NOTE
In this application note, we investigate the amplification and analysis of albumin protein derived from urine through the Platinum cutting-edge protein sequencing system. Albumin stands as a vital signpost for detecting illnesses, and urine acts as a hassle-free and immediate source for overseeing patient health. This guide presents a method that integrates affinity-centric amplification with Quantum-Si’s advanced protein sequencing to detect and profile albumin peptides.
Download the application note to uncover more about this innovative approach to studying biomarkers and protein discrepancies in bodily fluids.
Here, we depict the protein sequence of HSA following LysC digestion. These results highlight the precision of the Platinum workflow in correctly pinpointing and charting albumin peptides.
The patterns observed for each HSA peptide, as determined by the software analysis system, correspond to the fluorescence signals captured during sequencing. By scrutinizing these patterns, we could delve deeper into the sequencing data and confirm the precision of the peptide recognition.
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Immunoprecipitation of IL-6 from Human Serum for Next-Generation Protein Sequencing™ on Platinum – APP NOTE
The application note delves into the enrichment of low-abundant proteins from intricate biological matrices, a pivotal step in many proteomic workflows. It introduces an innovative immunoprecipitation protocol that aligns with Quantum-Si’s next-gen protein sequencing workflow. By bypassing the sample elution step, this on-bead digestion protocol directly prepares digested peptide libraries. The effectiveness of this method is showcased through the enrichment of IL-6 from human serum, followed by its sequencing and identification using Platinum.
Understand the potential of this groundbreaking immunoprecipitation protocol by downloading the full application note today.
The application note highlights a comprehensive workflow for on-bead digestion and immunoprecipitation of proteins, paving the way for next-generation protein sequencing™. This step-by-step process emphasizes the seamless transition from protein capture to its preparation for advanced sequencing, highlighting the efficiency and innovation of the approach.
Here we showcase the intricate details of the traces, coverage, and pulse duration data for the peptides identified in IL-6 immunoprecipitated from PBS. Utilizing five recognizers, the study successfully identifies 12 amino acids, leading to the recognition of three distinct peptides.
This compelling analysis presents the number of aligned IL-6 peptides discovered in each immunoprecipitated serum sample. This data not only underscores the efficiency of the method but also offers insights into the relationship between input concentration and sequencing outcomes.
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Identifying Monoclonal Antibodies with Quantum-Si’s Next-Generation Protein Sequencing™ Technology – APP NOTE
Identifying low-abundance monoclonal antibodies within a polyclonal antibody population is crucial for understanding disease mechanisms, diagnostics, and more. Traditional methods, while useful, often come with high costs, complexity, and time-consuming procedures. This application note introduces Quantum-Si’s next-generation protein sequencing™ technology on the Platinum instrument, a solution that addresses these challenges and efficiently identifies these critical antibodies.
Discover the breakthrough methodology and its potential impact by downloading the full application note.
Here we showcase the sequencing data of unique peptides derived from two distinct monoclonal antibodies. The data, collected over a 10-hour period, displays the recognition segments (RS) with their coverage indicated by the color height in each box, alongside the pulse duration distribution. This visualization effectively demonstrates the Platinum instrument’s capability to accurately sequence and identify specific peptides unique to each antibody.
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Characterization of Serum Proteins by Coupling Protein Separation Techniques with Next-Generation Protein Sequencing™ on Platinum®
Proteins play a vital role in virtually all biological processes, serving as key regulators of cellular functions, disease biomarkers, and therapeutic targets. However, the identification, isolation, and characterization of proteins from biological specimens can be challenging due to their complexity and diversity. Next-Generation Protein Sequencing (NGPS) on Platinum® offers a groundbreaking approach to deeply interrogate individual proteins with high resolution on a user-friendly benchtop platform. When combined with protein separation or enrichment methods, Platinum enables characterization of individual proteins from biological samples, revealing crucial details such as amino acid variants and PTMs. In this application note, we demonstrate a method that combines Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein separation and NGPS on Platinum to identify and characterize serum proteins. This method holds potential for applications beyond serum, offering new insights into disease mechanisms and advancing therapeutic and diagnostic developments.
Download the full application note to learn more.