Uncover New Insights with Post‑translational Modification (PTM) Analysis
Interrogate PTMs and proteoforms with ease on Platinum® Pro’s Next-Generation Protein Sequencing™ (NGPS™) platform, now powered by ProteoVue™ protein variant informatics workflow.
See ProteoVue PaperWhy choose Next-Generation Protein Sequencing for PTMs?
Proteoforms, such as PTMs, play crucial roles in biological and disease mechanisms.
Traditional proteomics techniques such as MS often struggle to capture the full diversity and complexity of proteoforms. NGPS leverages single-molecule sequencing to directly analyze individual protein molecules, providing detailed information on their modi cations and variation. This capability enables previously unattainable methods of protein study, paving the way for new discoveries in biology and medicine.
Generate deeper insights.
Discover more about your proteins. Platinum Pro technology enables you to discover new insights into biological function with the capability to conduct analysis of PTMs. Proteoform research is critical in identifying protein variations associated with phenotypic diversity and understanding complex disease mechanisms. Researchers are actively exploring the human proteome to uncover each protein’s unique function and its contribution to biological processes, disease mechanisms, and cellular interactions.
Quantum-Si invites researchers and potential collaborators to experience the ProteoVue workflow. Join the community of pioneers seeking deeper understanding and groundbreaking discoveries.
Next-Generation Protein Sequencing enables you to:
Recognize PTM events with unique kinetic signatures
Explore proteoforms at single-molecule resolution
Quantify protein variation at the amino acid level
See how Platinum Pro’s single-molecule resolution overcomes obstacles presented by other PTM analysis methods:
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Detecting Amino Acid Variants Using Next-Generation Protein Sequencing (NGPS) — PREPRINT PUBLICATION
Next-Generation Protein Sequencing (NGPS) is a single-molecule approach for characterizing protein variants, offering detailed insight into proteoforms and amino acid substitutions not easily discerned by mass spectrometry. The novel data type produced by NGPS, which is based on binding of N-terminal amino acids by fluorescently tagged recognizer proteins, requires the development of new data analysis methods and bioinformatic tools.
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Detection and Differentiation of PTMs with Platinum – APP NOTE
Arginine PTM analysis
Arginine plays a critical role in protein structure and function and often mediates key interactions between protein binding partners or between proteins and DNA. The two most common arginine PTMs are citrullination and dimethylation, which respectively alter arginine into citrulline or ADMA and SDMA.
ADMA and SDMA isomers share structural similarities and an identical mass, limiting their identification through traditional mass spectrometry. Likewise, determining arginine to citrulline results in a negligible mass increase. Another common method for PTM detection is antibody-based immunoassay ELISA, but only a few antibodies against arginine PTMs are commercially available, restricting its application.
Direct detection of arginine PTMs in proteins with Platinum
With Platinum’s single-molecule resolution and sensitivity, you can detect and differentiate arginine PTMs accurately. Peptide fragments are probed in real-time by fluorescently labeled recognizers, designed to bind on and off to their cognate N-terminal amino acid (NAA), while aminopeptidases in solution remove analyzed NAAs to expose subsequent amino acids for further recognition.
Fluorescence lifetime, intensity, and kinetic data are collected in real-time and analyzed to determine amino acid sequence and PTM content. This novel single-molecule sequencing approach can overcome the challenges facing conventional methods through unparalleled amino acid detection.Using Quantum-Si’s single-molecule protein sequencing, we demonstrated how to detect citrulline and how to differentiate between dimethylated arginine residues efficiently.
Platinum Can Detect and Differentiate PTMs – Download the application note to see how the process works and the PTM analysis that Platinum® delivers. -
Beyond the Genome: Identification of Protein Sequence and PTMs with Quantum-Si’s Next- Generation Protein Sequencer – POSTER
To demonstrate the versatility of Platinum and the use of kinetic signatures, we sequenced various types of samples, including mixtures of recombinant proteins, peptides with post-translational modifications (PTMs), proteins immunoprecipitated from human serum, and proteins isolated from human serum via fractionation with SDS-PAGE. The resulting peptides generated distinct kinetic signatures aligned to their respective sequences, highlighting the efficacy of Quantum-Si’s sequencing platform in analyzing multi-protein mixtures at reduced input concentrations.
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Beyond the Genome: Unraveling Protein Variability with Quantum-Si’s Next-Generation Protein Sequencing Technology – POSTER
Protein sequencing is a groundbreaking advancement in proteomics that augments genomics and transcriptomics research by providing crucial insights into the functional proteins encoded by the genome. Protein sequencing offers a more complete understanding of cellular processes and disease mechanisms by detecting changes at the protein level, such as post-translational modifications (PTMs), which cannot be captured by genomics data alone. Next-Generation Protein Sequencing™ (NGPS) on Platinum® enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument.